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- Title
Combined effects of some chemicals on β-galactosidase activity using a new semiparametric errors in variables model.
- Authors
YALAZ, Seçil; MATPAN BEKLER, Fatma; ACER, Ömer
- Abstract
The combined effects of some chemicals on β--galactosidase activity by Anoxybacillus sp. FMB1 were studied using semiparametric errors in variables methodology when the error has an unknown distribution. An experimental design was chosen to explain six chemicals, dithiothreitol (DTT), phenylmethylsulfonyl fluoride (PMSF), N--ethylmaleimide (NEM), iodoacetamide (Iod A),β-Mercaptoethanol (β-Mer) and 1,10-phenanthroline (phen) and to investigate the results. This technique constrained the number of real experiments performed while considering conceivable interactions between six chemicals. The optimal combinations of chemical concentration for maximum β-galactosidase activity was determined as 8 mM DTT, 8 mM PMSF, 8 mM NEM, 8 mM Iod A, 2 mM β-Mer and 2 mM phen. We also compared the no measurement error case and errors in variables case. The proposed estimator shown has better performances.
- Subjects
ERRORS-in-variables models; REAL numbers; ACRYLONITRILE; MEASUREMENT errors
- Publication
Banat's Journal of Biotechnology, 2019, Vol 10, Issue 19, p42
- ISSN
2068-4673
- Publication type
Article
- DOI
10.7904/2068-4738-X(19)-42