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- Title
Assigning the Sex-Specific Markers via Genotyping-by-Sequencing onto the Y Chromosome for a Torrent Frog Amolops mantzorum.
- Authors
Luo, Wei; Xia, Yun; Yue, Bisong; Zeng, Xiaomao
- Abstract
We used a genotyping-by-sequencing (GBS) approach to identify sex-linked markers in a torrent frog (Amolops mantzorum), using 21 male and 19 female wild-caught individuals from the same population. A total of 141 putatively sex-linked markers were screened from 1,015,964 GBS-tags via three approaches, respectively based on sex differences in allele frequencies, sex differences in heterozygosity, and sex-limited occurrence. With validations, 69 sex-linked markers were confirmed, all of which point to male heterogamety. The male specificity of eight sex markers was further verified by PCR amplifications, with a large number of additional individuals covering the whole geographic distribution of the species. Y chromosome (No. 5) was microdissected under a light microscope and amplified by whole-genome amplification, and a draft Y genome was assembled. Of the 69 sex-linked markers, 55 could be mapped to the Y chromosome assembly (i.e., 79.7%). Thus, chromosome 5 could be added as a candidate to the chromosomes that are particularly favored for recruitment in sex-determination in frogs. Three sex-linked markers that mapped onto the Y chromosome were aligned to three different promoter regions of the Rana rugosaCYP19A1 gene, which might be considered as a candidate gene for triggering sex-determination in A. mantzorum.
- Subjects
Y chromosome; FROGS; GENE mapping; GENETIC markers; SEX-linkage (Genetics)
- Publication
Genes, 2020, Vol 11, Issue 7, p727
- ISSN
2073-4425
- Publication type
Article
- DOI
10.3390/genes11070727