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- Title
Involvement of GPR43 Receptor in Effect of Lacticaseibacillus rhamnosus on Murine Steroid Resistant Chronic Obstructive Pulmonary Disease: Relevance to Pro-Inflammatory Mediators and Oxidative Stress in Human Macrophages.
- Authors
Sá, Ana Karolina; Olímpio, Fabiana; Vasconcelos, Jessica; Rosa, Paloma; Faria Neto, Hugo Caire; Rocha, Carlos; Camacho, Maurício Frota; Barcick, Uilla; Zelanis, Andre; Aimbire, Flavio
- Abstract
Background: Cytokine storm and oxidative stress are present in chronic obstructive pulmonary disease (COPD). Individuals with COPD present high levels of NF-κB-associated cytokines and pro-oxidant agents as well as low levels of Nrf2-associated antioxidants. This condition creates a steroid-resistant inflammatory microenvironment. Lacticaseibacillus rhamnosus (Lr) is a known anti-cytokine in lung diseases; however, the effect of Lr on lung inflammation and oxidative stress in steroid-resistant COPD mice remains unknown. Objective: Thus, we investigated the Lr effect on lung inflammation and oxidative stress in mice and macrophages exposed to cigarette smoke extract (CSE) and unresponsive to steroids. Methods: Mice and macrophages received dexamethasone or GLPG-094 (a GPR43 inhibitor), and only the macrophages received butyrate (but), all treatments being given before CSE. Lung inflammation was evaluated from the leukocyte population, airway remodeling, cytokines, and NF-κB. Oxidative stress disturbance was measured from ROS, 8-isoprostane, NADPH oxidase, TBARS, SOD, catalase, HO-1, and Nrf2. Results: Lr attenuated cellularity, mucus, collagen, cytokines, ROS, 8-isoprostane, NADPH oxidase, and TBARS. Otherwise, SOD, catalase, HO-1, and Nrf2 were upregulated in Lr-treated COPD mice. Anti-cytokine and antioxidant effects of butyrate also occurred in CSE-exposed macrophages. GLPG-094 rendered Lr and butyrate less effective. Conclusions: Lr attenuates lung inflammation and oxidative stress in COPD mice, suggesting the presence of a GPR43 receptor-dependent mechanism also found in macrophages.
- Subjects
BIOLOGICAL models; STEROIDS; MACROPHAGES; INFLAMMATORY mediators; OXIDATIVE stress; LUNGS; DESCRIPTIVE statistics; MICE; EXPERIMENTAL design; OBSTRUCTIVE lung diseases; LACTOBACILLUS; ANIMAL experimentation; BUTYRIC acid; CYTOKINES; SMOKE; CELL receptors; DEXAMETHASONE; PHARMACODYNAMICS
- Publication
Nutrients, 2024, Vol 16, Issue 10, p1509
- ISSN
2072-6643
- Publication type
Article
- DOI
10.3390/nu16101509