We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Intracerebroventricular LGI1 antibody administration increase seizure severity and impair memory in rats.
- Authors
Pişkin, Şura Akat; Yüceer, Hande; Ulusoy, Canan; Soylu, Selen; Karaaslan, Zerrin; Turgeon, Ece Erdağ; Yılmaz, Nihan Çarçak; Küçükali, Cem İsmail; Onat, Filiz; Tüzün, Erdem
- Abstract
Objective: Anti-LGI1 encephalitis is a neuroimmunological syndrome associated with memory impairment and frequent seizures. In this study, it was aimed to clarify the role of LGI antibodies on epileptogenesis and cognitive performance in the passive transfer rat model. Methods: IgG purified from the peripheral blood of anti-LGI encephalitis patients (n=10) and healthy controls (HC) (n=9), and serum physiologic (SF) (n=7) were intracerebroventricularly injected into non-epileptic Wistar rats on consecutive days. Behavioral assessments and EEG recordings were performed before and after IgG administration. A convulsive dose (45 mg/kg) of pentylenetetrazol (PTZ) was then intraperitoneally administered. Acutely induced epileptic discharges and seizure stages were evaluated and compared between groups. Results: Motor seizure latency, first motor seizure duration, and latency for myoclonus were increased in LGI1 group. In addition, LGI1 antibody-given rats had significantly higher stage of seizures compared to HC and SF groups (p<0.05). Also, vertical activity in open field maze, spontaneous alternation in Y-maze, and discrimination index in novel object recognition test were significantly different in LGI1 group (p<0.05). Conclusion: LGI1 antibodies appear to increase the susceptibility of seizures as well as disrupt memory functions. Here developed LGI1 antibody-mediated passive transfer autoimmune encephalitis rat model can be considered as a potential in-vivo model for anti-LGI1 encephalitis. Further work will elucidate the complex role of LGI1 antibodies in seizures and cognitive deficits observed in anti-LGI1 encephalitis.
- Subjects
SEIZURES (Medicine); EPILEPSY; IMMUNOGLOBULINS; BEHAVIORAL assessment; MEMORY disorders; COGNITIVE ability
- Publication
Anatomy: International Journal of Experimental & Clinical Anatomy, 2022, Vol 16, p227
- ISSN
1307-8798
- Publication type
Abstract
- DOI
10.2399/ana.22.003s