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- Title
MicroRNA-146a-5p and microRNA‐210‐3p Correlate with T Regulatory Cells Frequency and Predict Asthma Severity in Egyptian Pediatric Population.
- Authors
Eldosoky, Mona A; Hammad, Reham; Rushdi, Areej; Ibrahim, Hanan F; Tawfeik, Amany M; Mora, Ahmed; Fahmy, Sarah F; El-Ashmawy, Hossam; Ali, Elham; Hamed, Dina H; Mohammed, Amena Rezk; Mashaal, Alya; Mohsen, Hanan
- Abstract
Background: Severe bronchial asthma (BA) affects 5– 10% of children, which imposes socioeconomic burden. Therefore, it is crucial to identify biomarkers for risk stratification in children with BA. T regulatory cells (Tregs) play a balancing role in allergic response regulation. We aimed to investigate the relationship between Treg, miR-210-3p, and miR-146a-5p in relation to asthma phenotypes in search of novel biomarkers of disease severity. Methods: This study included 50 children with BA classified into Group 1 (n = 25) children with mild to moderate asthma and Group 2 (n = 25) children with severe asthma. In addition to 26 control subjects. Flow cytometry was used to detect Tregs. Plasma miR-210-3p and miR-146a levels were determined using quantitative real-time PCR. Patients' FEV1 (Forced Expiratory Volume in the first second) was measured. Results: miR-210-3p level correlated negatively with Treg frequency (r = − 0.828, P < 0.001) and FEV1 (r = − 0.621, P < 0.001). The level of miR-146a-5p positively correlated positively with Treg% (r = 0.303, P = 0.032). ROC curve analysis revealed that miR-210-3p was the most sensitive biomarker of severity, with the area under curve (AUC) = 0.923, 96% sensitivity, and 60% specificity. According to multivariate analysis, miR-210-3p is an independent risk factor for BA severity [OR =3.119, P = 0.030], while miR-146a-5p is a protective factor [OR =0.811, P = 0.049]. Conclusion: Treg frequency is linked to FEV1, miR-146a-5p and miR-210-3p in childhood BA. Upregulation of miR-210-3p is a sensitive biomarker and an independent risk factor for BA severity in Egyptian children.
- Subjects
REGULATORY T cells; CHILD patients; EGYPTIANS; ASTHMA; ASTHMA in children
- Publication
Journal of Asthma & Allergy, 2023, Vol 16, p107
- ISSN
1178-6965
- Publication type
Article
- DOI
10.2147/JAA.S398494