We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Recent Advancements in Reducing the Off-Target Effect of CRISPR-Cas9 Genome Editing.
- Authors
Mengstie, Misganaw Asmamaw; Azezew, Muluken Teshome; Dejenie, Tadesse Asmamaw; Teshome, Assefa Agegnehu; Admasu, Fitalew Tadele; Teklemariam, Awgichew Behaile; Mulu, Anemut Tilahun; Agidew, Melaku Mekonnen; Adugna, Dagnew Getnet; Geremew, Habtamu; Abebe, Endeshaw Chekol
- Abstract
The CRISPR-Cas (Clustered Regularly Interspaced Short Palindromic Repeat (CRISPR)) and the associated protein (Cas9) system, a young but well-studied genome-editing tool, holds plausible solutions to a wide range of genetic disorders. The single-guide RNA (sgRNA) with a 20-base user-defined spacer sequence and the Cas9 endonuclease form the core of the CRISPR-Cas9 system. This sgRNA can direct the Cas9 nuclease to any genomic region that includes a protospacer adjacent motif (PAM) just downstream and matches the spacer sequence. The current challenge in the clinical applications of CRISPR-Cas9 genome-editing technology is the potential off-target effects that can cause DNA cleavage at the incorrect sites. Off-target genome editing confuses and diminishes the therapeutic potential of CRISPR-Cas9 in addition to potentially casting doubt on scientific findings regarding the activities of genes. In this review, we summarize the recent technological advancements in reducing the off-target effect of CRISPR-Cas9 genome editing.
- Subjects
GENOME editing; CRISPRS; ENDONUCLEASES; TECHNOLOGICAL innovations; GENETIC disorders; RNA
- Publication
Biologics: Targets & Therapy, 2024, Vol 18, p21
- ISSN
1177-5475
- Publication type
Article
- DOI
10.2147/BTT.S429411