We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
MHC-IIB Filament Assembly and Cellular Localization Are Governed by the Rod Net Charge.
- Authors
Rosenberg, Michael; Straussman, Ravid; Ben-Ya'acov, Ami; Ronen, Daniel; Ravid, Shoshana
- Abstract
Background. Actin-dependent myosin II molecular motors form an integral part of the cell cytoskeleton. Myosin II molecules contain a long coiled-coil rod that mediates filament assembly required formyosin II to exert its full activity. The exact mechanisms orchestrating filament assembly are not fully understood. Methodology/Principal Findings. Here we examine mechanisms controlling filament assembly of non-muscle myosin IIB heavy chain (MHC-IIB). We show that in vitro the entire C-terminus region of net positive charge, found in myosin II rods, is important for self-assembly of MHC-IIB fragments. In contrast, no particular sequences in the rod region with net negative charge were identified as important for self-assembly, yet a minimal area from this region is necessary. Proper paracrystal formation by MHC-IIB fragments requires the 196aa charge periodicity along the entire coiled-coil region. In vivo, in contrast to self-assembly in vitro, negatively-charged regions of the coiled-coil were found to play an important role by controlling the intracellular localization of native MHC-IIB. The entire positively-charged region is also important for intracellular localization of native MHC-IIB. Conclusions/Significance. A correct distribution of positive and negative charges along myosin II rod is a necessary component in proper filament assembly and intracellular localization of MHC-IIB.
- Subjects
MAJOR histocompatibility complex genetics; MYOSIN; MUSCLE proteins; CELLULAR immunity; IMMUNE response; CELLULAR mechanics; IMMUNOGENETICS; HUMAN immunogenetics; IMMUNOLOGY
- Publication
PLoS ONE, 2008, Vol 3, Issue 1, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0001496