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- Title
The effects of sperm preparation media on motility, viability and DNA integrity of human spermatozoa.
- Authors
Anbari F.; Halvaei I.; Nabi A.; Ghazali Sh.; Khalili M. A.; Johansson L.
- Abstract
Introduction: The main goal was to compare the effects of three different sperm preparation media on sperm motility, viability and DNA integrity of semen samples from normozoospermic men. Materials and Methods: A total of 15 normozoo spermic males were included in the study. The semen analysis was performed in accordance with the WHO guidelines (2010). After semen analysis, each sample was divided into three aliquots and swim-up performed with three different sperm preparation media (Sperm Preparation Media, Origio, Denmark, Ham's F10, Biochrome, Berlin, Germany, and VitaSpermTM, Innovative Biotech, Iran). Sperm motility, viability and DNA fragmentation,were evaluated at 0, 1, 2, and 24 hr after swim-up. Results: There were no significant differences, at any time intervals, in the totalsperm motility between the different sperm preparation media. However, the rate of progressive motility was significantly higher in spermatozoa prepared by the media from Origio in comparison to VitaSperm TM (68.3±11.5 vs. 58±9.8, p=0.03), whereas no significant difference was found against Ham's F10. No significant differences in sperm viability were seen between the media products .One hour after swim-up therate of sperm DNA fragmentation was also significantly lower inthe medium from Origio versus VitaSpermTM (p=0.02). Conclusion: The type of medium for preparation of semen samples from normozoospermic men significantly affects the performance of spermatozoain assisted conception.
- Subjects
SEMEN analysis; SPERMATOZOA analysis; SPERM motility; ALIQUOTS (Chemistry); GUIDELINES; WORLD Health Organization
- Publication
Iranian Journal of Reproductive Medicine, 2015, p31
- ISSN
1680-6433
- Publication type
Article