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- Title
Pathogenicity Studies with the Fungi , , and : Induction of Skin Ulcers in Striped Mullet.
- Authors
Sosa, Emilio R.; Landsberg, Jan H.; Kiryu, Yasunari; Stephenson, Christy M.; Cody, Theresa T.; Dukeman, Angela K.; Wolfe, Heather P.; Vandersea, Mark W.; Litaker, R. Wayne
- Abstract
Based on isolations from naturally infected fish in Florida, we investigated the role of the fungi , , and in the etiology of ulcerative mycosis (UM) in striped mullet . We injected healthy striped mullet subcutaneously with secondary zoospores of four oomycete isolates: two concentrations (50 and 115 zoospores/mL) of SJR (an endemic isolate of in American shad from the St. Johns River); two concentrations each of CAL (25 and 65 zoospores/mL) and ACH (1,400 and 2,000 zoospores/mL; endemic isolates of and , respectively, in striped mullet from the Caloosahatchee River); and two concentrations of the ascomycete culture MTZ (2,500 and 3,500 zoospores/mL; endemic isolate of from whirligig mullet in the Matanzas Inlet). All fish injected with either concentration of SJR developed granulomatous ulcers after 8 d and died within 21 d. Eighty percent (8/10) of fish injected with the high dose of CAL developed ulcers after 13 d and died within 28 d, but only 30% (3/10) of fish injected with the low dose of CAL developed ulcers. Four of the ulcerated fish died within 28 d, and the remaining fish were terminated after 32 d. Fish injected with zoospores of developed ulcers that were grossly and histologically similar to those observed in naturally infected striped mullet with UM from several estuaries or rivers in Florida. These hemorrhagic skin ulcers were characterized by myonecrosis and the presence of mycotic granulomas. None of the fish injected with ACH, MTZ, or sterile water developed ulcers. This study fulfilled Koch's postulates and demonstrated that ulcers could be experimentally induced in striped mullet after exposure via injection to secondary zoospores of an endemic Florida strain of .
- Publication
Journal of Aquatic Animal Health, 2007, Vol 19, Issue 1, p41
- ISSN
0899-7659
- Publication type
Article
- DOI
10.1577/H06-013.1