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- Title
Osteoblastic potential of infrapatellar fat pad-derived mesenchymal stem cells from rheumatoid arthritis and osteoarthritis patients.
- Authors
Skalska, Urszula; Prochorec‐Sobieszek, Monika; Kontny, Ewa
- Abstract
Aim To evaluate the osteoblastic potential of adipose-derived mesenchymal stem cells ( ASCs) from infrapatellar fat pad ( IPFP) of rheumatoid arthritis ( RA) patients in comparison to osteoarthritis ( OA) patients, as well as the influence of tumor necrosis factor alpha ( TNFα) on osteoblastic ASC differentiation in vitro. Methods ASCs were isolated from IPFP of RA and OA patients. After expansion, cells were cultured in osteogenic medium with or without TNFα. After 2 weeks, expression of BMP-2, Runx-2, osterix ( Osx), collagen 1a1 ( Col1a1) and osteopontin ( OPN) messenger RNA (m RNA) was assessed by reverse transcription polymerase chain reaction and calcium deposition by alizarin red staining. Dickkopf-1 ( DKK-1) and osteoprotegerin ( OPG) protein concentrations were measured in culture supernatants using enzyme-linked immunosorbent assay. Results Both RA- and OA- ASCs cultured in osteogenic medium showed calcium deposition. The expression of Runx2 and OPN m RNA was increased in RA- ASCs. These cells expressed significantly more Osx and OPN than OA- ASCs. TNFα potentiated calcium deposition, up-regulated Runx2 and BMP-2 but down-regulated Col1a1 and OPN expression. In osteogenic cultures DKK-1 concentration was increased but that of OPG decreased, whereas TNFα elevated secretion of both cytokines. Conclusion RA- ASCs have comparable or slightly stronger osteogenic potential than OA- ASCs. RA- ASCs seem to be more sensitive to TNFα treatment. TNFα exerts complex effects on ASC osteoblastogenesis, enhances expression of early osteogenic markers and calcium deposition, inhibits expression of m RNA coding for non-mineral bone components and alters ASC secretory activity.
- Subjects
MESENCHYMAL stem cells; RHEUMATOID arthritis; OSTEOARTHRITIS; OSTEOPROTEGERIN; ENZYME-linked immunosorbent assay
- Publication
International Journal of Rheumatic Diseases, 2016, Vol 19, Issue 6, p577
- ISSN
1756-1841
- Publication type
Article
- DOI
10.1111/1756-185X.12368