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- Title
Chiral purity test of bevantolol by capillaryelectrophoresis and high performance liquid chromatography.
- Authors
Long, Pham; Trung, Tran; Oh, Joung; Kim, Kyeong
- Abstract
Two methods for the chiral purity determination of bevantolol were developed, namely capillary electrophoresis (CE) using carboxymethyl-β-cyclodextrin (CM-β-CD) as a chiral selector and high-perfomance liquid chromatography (HPLC) using a chiral stationary phase. In the HPLC method, the separation of bevantolol enantiomers was performed on a Chiralpak AD-H column by isocratic elution with n-hexane-ethanol-diethylamine (10:90:0.1, v/v/v) as mobile phase. In the CE method, bevantolol enantiomers were separated on an uncoated fused silica capillary with 50 mM amonium phosphate dibasic adjusted to a pH 6.5 with phosphoric acid containing 15 mM CM-β-CD as running buffer. Validation data such as linearity, recovery, detection limit, and precision of the two methods are presented. The detection limits of S-(−)-bevantolol were 0.1% and 0.05% for CE and HPLC method, respectively and R-(+)-bevantolol were 0.15% and 0.05% for CE and HPLC method, respectively. There was generally good agreement between the HPLC and CE results.
- Publication
Archives of Pharmacal Research, 2006, Vol 29, Issue 9, p808
- ISSN
0253-6269
- Publication type
Article
- DOI
10.1007/BF02974083