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- Title
Improving quantification of particulate phosphorus.
- Authors
Hu, Ying‐Yu; Irwin, Andrew J.; Finkel, Zoe V.
- Abstract
Total particulate phosphorus (TPP) is often determined using the high‐temperature dry combustion (HTDC) method followed by hydrolysis of the ash and then molybdenum colorimetry. Here, we show that a higher than traditionally used combustion temperature, 800°C vs. 450–550°C, improves phosphorus recovery from several phosphorus standard compounds, marine phytoplankton cultures, and particulate samples from the field. The ashing auxiliary MgSO4 further improves P recovery by improving decomposition, reducing volatilization during combustion, and increasing the efficiency of hydrolysis. A 0.2 M HCl hydrolysis, under 90°C for 30 min yields a higher P recovery compared with hydrolysis at room temperature or 60°C. In aggregate, these improvements to the method double the P recovery from phospholipids to 97%. TPP recovery from laboratory phytoplankton cultures and field samples increased an average of 11%, primarily due to the improvements in P recovery from phospholipids, polyphosphate, and nucleic acids. We refer to this new method as the eXtra high temperature dry combustion (X‐HTDC) Ash/Hydrol method and recommend its application for measuring particulate phosphorus from organic compounds in aquatic systems.
- Subjects
ORGANOPHOSPHORUS compounds; PHOSPHORUS in water; MARINE phytoplankton; PHOSPHORUS; PHOSPHORUS compounds; NUCLEIC acids; DOMOIC acid; PHYTASES
- Publication
Limnology & Oceanography, Methods, 2022, Vol 20, Issue 11, p729
- ISSN
1541-5856
- Publication type
Article
- DOI
10.1002/lom3.10517