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- Title
Physical mapping of chromosome 4J of Thinopyrum bessarabicum using gamma radiation-induced aberrations.
- Authors
Pu, Jing; Wang, Qing; Shen, Yuefeng; Zhuang, Lifang; Li, Chenxu; Tan, Mengfa; Bie, Tongde; Chu, Chenggen; Qi, Zengjun
- Abstract
Key message: Gamma radiation induced a series of structural aberrations involving Thinopyrum bessarabicum chromosome 4J. The aberrations allowed for deletion mapping of 101 4J-specific markers and fine mapping of blue-grained gene BaThb. Abstract: Irradiation can induce translocations and deletions to assist physically locating genes and markers on chromosomes. In this study, a 12-Gy dosage of Co-γ was applied to pollen and eggs of a wheat ( Triticum aestivum) landrace Chinese Spring (CS)- Thinopyrum bessarabicum chromosome 4J disomic addition line (DA4J), and the gametes from irradiated plants were fertilized with normal CS eggs or pollen to produce M seeds. Based on genomic in situ hybridization analysis of 261 M plants, we identified 74 lines carrying structural aberrations involving chromosome 4J with the higher aberration rate in treated pollen (31.2 %) than in the treated eggs (21.3 %). We further identified 43 (53.8 %) lines with structural aberrations on chromosome 4J by analyzing another 80 M plants with 74 4J-specific markers, indicating that combining molecular and cytological methods was more efficient for detecting chromosome aberrations. Marker analysis thus was performed prior to cytogenetic identification on M-M seeds to detect chromosome structural aberrations. Sixty-eight M lines with structural aberrations on chromosome 4J and six previously obtained chromosome 4J alien lines were then analyzed using 101 chromosome 4J-specific markers. After combining marker results with chromosome aberrations in each line, chromosome 4J was physically divided into 24 segmental blocks with 7 in the short arm and 17 in the long arm. The blue-grained gene BaThb was further mapped into the region corresponding to block 4JL-11. The chromosome aberrations and the physical map developed in this research provide useful stocks and tools for introgression of genes on chromosome 4J into wheat.
- Subjects
PLANT gene mapping; GRASSES; GAMMA rays; CHROMOSOME abnormalities; GENETIC markers in plants; POLLEN; PLANTS
- Publication
Theoretical & Applied Genetics, 2015, Vol 128, Issue 7, p1319
- ISSN
0040-5752
- Publication type
Article
- DOI
10.1007/s00122-015-2508-y