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- Title
Screening and identification of proteins interacting with Brassica napus heat shock protein 23.
- Authors
WANG Fang; LIU Chao; WU Jiang-sheng; ZHANG Zhong-ming
- Abstract
Yeast two-hybrid assay was carried out to screen the interaction proteins of Brassica napus heat shock protein Bnp23 using activator domain-cDNA library of Arabidopsis thaliana seedlings.39 positive clones were isolated;and among them,2 clones were encoded by Arabidopsis PYK10 binding protein 1(AtPBP1).In vitro interaction between AtPBP1 and Bnp23 was further confirmed by western blotting.An interaction between AtPBP1 homologous protein BnMBP6(myrosase binding protein 6 of B.napus) and Bnp23 was also identified by yeast two-hybrid assay.
- Subjects
HEAT shock proteins; ARABIDOPSIS; EDIBLE fungi; CARRIER proteins; FIRE assay
- Publication
Chinese Journal of Oil Crop Sciences, 2011, Vol 33, Issue 4, p318
- ISSN
1007-9084
- Publication type
Article