We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Anti-proliferative effect of extremely low frequency electromagnetic field on preneoplastic lesions formation in the rat liver.
- Authors
Jiménez-García, Mónica Noemí; Arellanes-Robledo, Jaime; Aparicio-Bautista, Diana Ivette; Rodríguez-Segura, Miguel Ángel; Villa-Treviño, Saúl; Godina-Nava, Juan José
- Abstract
Background: Recently, extremely low frequency electromagnetic fields (ELF-EMF) have been studied with great interest due to their possible effects on human health. In this study, we evaluated the effect of 4.5 mT - 120 Hz ELF-EMF on the development of preneoplastic lesions in experimental hepatocarcinogenesis. Methods: Male Fischer-344 rats were subjected to the modified resistant hepatocyte model and were exposed to 4.5 mT - 120 Hz ELF-EMF. The effects of the ELF-EMF on hepatocarcinogenesis, apoptosis, proliferation and cell cycle progression were evaluated by histochemical, TUNEL assay, caspase 3 levels, immunohistochemical and western blot analyses. Results: The application of the ELF-EMF resulted in a decrease of more than 50% of the number and the area of ?- glutamyl transpeptidase-positive preneoplastic lesions (P = 0.01 and P = 0.03, respectively) and glutathione Stransferase placental expression (P = 0.01). The number of TUNEL-positive cells and the cleaved caspase 3 levels were unaffected; however, the proliferating cell nuclear antigen, Ki-67, and cyclin D1 expression decreased significantly (P ⩽ 0.03), as compared to the sham-exposure group. Conclusion: The application of 4.5 mT - 120 Hz ELF-EMF inhibits preneoplastic lesions chemically induced in the rat liver through the reduction of cell proliferation, without altering the apoptosis process.
- Subjects
PRECANCEROUS conditions; ELF electromagnetic fields; APOPTOSIS; GLUTATHIONE; CELL proliferation; LABORATORY rats
- Publication
BMC Cancer, 2010, Vol 10, p159
- ISSN
1471-2407
- Publication type
Article
- DOI
10.1186/1471-2407-10-159