We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Stabilization and quantitative measurement of nicotinamide adenine dinucleotide in human whole blood using dried blood spot sampling.
- Authors
Matsuyama, Ryo; Omata, Tomoyo; Kageyama, Michiharu; Nakajima, Ryota; Kanou, Masanobu; Yamana, Kei
- Abstract
Nicotinamide adenine dinucleotide (NAD+) is a coenzyme essential for energy production. Recently, associations between NAD+ and aging-related diseases have been reported, and NAD+ precursors that increase NAD+ concentration in the body have been acknowledged as anti-aging supplements. However, there have been only a few studies on the link between aging or aging-related diseases and human blood NAD+ concentration because NAD+ and its precursors are unstable in blood and difficult to measure. Therefore, we aimed to construct a quantitative NAD+ measurement method that is simpler than the existing methods. The calibration standards of NAD+ showed good linearity (0.9936 to 0.9990) in the range of 0.25 to 200 μM, and the lower limit of quantification was 0.5 to 2 μM. We found that QIAcard FTA DMPK-B maintained NAD+ stability of 85% or more for at least 2 weeks at 4 °C and 1 week at room temperature using the dried blood spot method. Additionally, NAD+ stability in the blood extraction solution was more than 90% for 2 months. To our knowledge, there has been no report on a quantitative NAD+ measurement method in human whole blood that can be performed with as little as 5 μL of blood and can be easily implemented at both medical clinics and private homes. Our simple and convenient method has the potential to become the gold standard for NAD+ measurement in blood. It is expected to contribute to the acceleration of research on the correlation between aging or aging-related diseases and NAD+ concentration in human blood.
- Subjects
NAD (Coenzyme); DRIED blood spot testing; ADENINE; BLOOD sampling; NICOTINAMIDE
- Publication
Analytical & Bioanalytical Chemistry, 2023, Vol 415, Issue 5, p775
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-022-04469-7