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- Title
Annotation and quantification of N-acyl homoserine lactones implied in bacterial quorum sensing by supercritical-fluid chromatography coupled with high-resolution mass spectrometry.
- Authors
Hoang, Thi Phuong Thuy; Barthélemy, Morgane; Lami, Raphaël; Stien, Didier; Eparvier, Véronique; Touboul, David
- Abstract
In recent years, use of supercritical-fluid chromatography (SFC) with CO2 as the mobile phase has been expanding in the research laboratory and industry since it is considered to be a green analytical method. This technique offers numerous advantages, such as good separation and sensitive detection, short analysis times, and stability of analytes. In this study, a method for quantification of N-acyl homoserine lactones (AHLs), signaling molecules responsible for cell-to-cell communication initially discovered in bacteria, by SFC coupled with high-resolution mass spectrometry (HRMS) was developed. The SFC conditions and MS ionization settings were optimized to obtain the best separation and greatest sensitivity. The optimal analysis conditions allowed quantification of up to 30 AHLs in a single run within 16 min with excellent linearity (R2 > 0.998) and sensitivity (picogram level). This method was then applied to study AHL production by one Gram-negative endophytic bacterium, Paraburkholderia sp. BSNB-0670. Nineteen known AHLs were detected, and nine abundant HSLs were quantified. To further investigate the production of uncommon AHLs, a molecular networking approach was applied on the basis of the SFC–HRMS/MS data. This led to additional identification of four unknown AHLs annotated as N-3-hydroxydodecanoylol homoserine lactone, N-3-hydroxydodecadienoyl homoserine lactone, and N-3-oxododecenoyl homoserine lactones (two isomers).
- Subjects
SUPERCRITICAL fluid chromatography; QUORUM sensing; MASS spectrometry; LACTONES; CHROMATOGRAPHIC analysis; ENDOPHYTIC bacteria
- Publication
Analytical & Bioanalytical Chemistry, 2020, Vol 412, Issue 10, p2261
- ISSN
1618-2642
- Publication type
Article
- DOI
10.1007/s00216-019-02265-4