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- Title
Nitratireductor soli sp. nov., isolated from phenol-contaminated soil.
- Authors
Chen, Qing; Ni, Hai-Yan; Zhuang, Wen; Sun, Zhong-Guan; Yang, Zhen-Zhen; Wang, Hong-Mei; He, Qin; He, Jian
- Abstract
Strain ZZ-1, a Gram-negative, rod-shaped bacterium, motile by flagella, was isolated from phenol-contaminated soil. Strain ZZ-1 was found to grow at 15-37 °C (optimum 25-30 °C), at pH 6.0-10.0 (optimum pH 7.5) and with 0-8.0 % (w/v) NaCl (optimum 0.5 %). The isolate was found to be able to reduce nitrate to nitrite, but not to nitrogen. Phylogenetic analysis based on 16S rRNA gene sequences indicated that strain ZZ-1 is a member of the genus Nitratireductor, and shows high sequence similarities to Nitratireductor pacificus MCCC 1A01024 (98.5 %) and lower (<97 %) sequence similarities to all other Nitratireductor species. Chemotaxonomic analysis revealed that strain ZZ-1 possesses Q-10 as the predominant ubiquinone and Summed feature 8(C ω6 c and/or C ω7 c; 66.6 %), C ω8 c cyclo (23.3 %), C (3.4 %), iso-C (2.3 %) and C (1.0 %) as the major fatty acids. The polar lipids of strain ZZ-1 were determined to be diphosphatidylglycerol, phosphatidylcholine, phospholipids, aminolipids, a glycolipid and an aminophospholipid. The DNA G+C content was determined to be 64.1 mol%. Based on the draft genome sequence, the DNA-DNA hybridization estimate value between strain ZZ-1 and N. pacificus MCCC 1A01024 was 46.5 ± 3.0 % and ANI was 75.9 %. The combination of phylogenetic analysis, phenotypic characteristics, chemotaxonomic data and DNA-DNA hybridization supports the conclusion that strain ZZ-1 represents a novel species of the genus Nitratireductor, for which the name Nitratireductor soli sp. nov. is proposed. The type strain is ZZ-1 (=JCM 30640 = MCCC 1K00508).
- Subjects
SOIL microbiology; GRAM-negative bacteria; BACTEROIDACEAE; NUCLEOTIDE sequencing; CHEMOTAXONOMY; SOIL pollution
- Publication
Antonie van Leeuwenhoek, 2015, Vol 108, Issue 5, p1139
- ISSN
0003-6072
- Publication type
Article
- DOI
10.1007/s10482-015-0567-3