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- Title
Unique human CD133<sup>+</sup> leukemia cell line and its modulation towards a mesenchymal phenotype by FGF2 and TGFβ<sub>1</sub><FNR></FNR><FN>Maria Bodo and Tiziano Baroni have contributed equally to this study. </FN>.
- Authors
Bodo, Maria; Baroni, Tiziano; Bellucci, Catia; Lilli, Cinzia; De Ioanni, Maria; Bonifacio, Elisabetta; Moretti, Lorenzo; Becchetti, Ennio; Bellocchio, Silvia; Delfini, Costante; Lumare, Eleonora; Tabilio, Antonio
- Abstract
Immunological features of GM-490 cells, a new blood cell line from a patient with acute lymphoblastic leukemia, included lack of CD34, CD38, CD45, CD14, HLA-DR, and lymphoid and myeloid markers and expression of CD29, CD36, CD44, CD54, CD71, CD105, and CD133. Molecular analysis indicated CD45 gene expression was absent but CD34 mRNA was present. GM-490 cells constitutively produced fibronectin (FN), type III and traces of type I collagen, collagenases, glycosaminoglycans (GAG) and biglycan and betaglycan proteoglycans (PG) as well as FGF2 and TGFβ1. When FGF2 and/or TGFβ1 were added to cells in vitro, they stimulated cell proliferation and differently modulated matrix production and growth factor receptor expression. Reverse transcription-polymerase chain reaction (RT-PCR) detection of transcripts encoding for osteocalcin and RUNX2 suggests GM-490 cells differentiate towards the osteoblast pathway. GM-490 cells expressed the low affinity nerve growth factor receptor (p75LNGFR), a somatic stem cell marker that is not detected in hematopoietic cells, leading to the hypothesis that GM-490 has mesenchymal stem cell properties. The reciprocal modulating effects of FGF2 and TGFβ1 on each other's receptors make the GM-490 cell line a new model for investigating the relationship between these growth factors and their receptors in autocrine loops which are believed to sustain the malignant clone in hematological diseases. © 2005 Wiley-Liss, Inc.
- Subjects
LYMPHOBLASTIC leukemia; CELL lines; MESSENGER RNA; FIBRONECTINS; GLYCOSAMINOGLYCANS; PROTEOGLYCANS; POLYMERASE chain reaction; NERVE growth factor
- Publication
Journal of Cellular Physiology, 2006, Vol 206, Issue 3, p682
- ISSN
0021-9541
- Publication type
Article
- DOI
10.1002/jcp.20517