We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Detection of thyroid-stimulating hormone receptor and G<sub>s</sub><sub>α</sub> mutations: in 75 toxic thyroid nodules by denaturing gradient gel electrophoresis.
- Authors
Trülzsch, Barbara; Krohn, Knut; Wonerow, Peter; Chey, Soroth; Holzapfel, Hans-Peter; Ackermann, Frank; Führer, Dagmar; Paschke, Ralf
- Abstract
The actual frequency of constitutively activating thyrotropin receptor or Gsα mutations in toxic thyroid nodules (TTNs) remains controversial as considerable variation in the prevalence of these mutations has been reported. We studied a series of 75 consecutive TTNs and performed mutation screening by the more sensitive method of denaturing gradient gel electrophoresis (DGGE) in addition to direct sequencing. Furthermore, the likelihood of somatic mutations occurring in genes other than that for the thyroid-stimulating hormone receptor (TSHR) and exons 7–9 of the Gsα protein gene was determined by clonality analysis of TTNs, which did not harbor mutations in the investigated genes. In 43 of 75 TTNs (57%) constitutively active TSHR mutations were identified. Six TSHR mutations were detected only by DGGE, underlining the importance of a sensitive screening method. Novel, constitutively activating mutations were identified at positions 425 (Ser→Leu) and 512 (Leu→Glu/Arg). Furthermore, a new base substitution was detected at position Pro639Ala (CCA→GCA). Ten of 20 TSHR or Gsα mutation negative cases (50%) showed nonrandom X-chromosome inactivation, indicating clonal origin. In conclusion, somatic, constitutively activating TSHR mutations appear to be a major cause of TTNs (57%), while mutations in Gsα play a minor role (3%). The mutation negative but clonal cases indicate a probable involvement of somatic mutations other than in the TSH receptor or Gsα genes as the molecular cause of these hot nodules.
- Subjects
GENETIC mutation; GENES; DIAGNOSIS; DRUG receptors; COLLOIDS; GENETICS
- Publication
Journal of Molecular Medicine, 2001, Vol 78, Issue 12, p684
- ISSN
0946-2716
- Publication type
Article
- DOI
10.1007/s001090000170