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- Title
Decreased MicroRNA-221 is Associated with High Levels of TNF-α in Human Adipose Tissue-Derived Mesenchymal Stem Cells From Obese Woman.
- Authors
Chou, Wen-Wen; Wang, Yu-Ting; Liao, Yi-Chu; Chuang, Shih-Chang; Wang, Shen-Nien; Juo, Suh-Hang Hank
- Abstract
Aim: The present study aimed to investigate the regulation and involvement of miR-221 in the differentiation of human adipose tissue-derived mesenchymal stem cells (hASCs). The relationships between miR-221 and pro-inflammatory markers and adipokines were also explored. Methods: Eight adipose tissues were obtained from four obese (mean body mass index (BMI) =31.7 kg/m2) and four lean (mean BMI= 21.5 kg/m2) women. hASCs were induced to differentiate, and the related gene expression were measured in the hASC-differentiated adipocytes using real-time reverse transcriptase polymerase chain reaction (real-time RT-PCR). Results: During adipogenesis, miR-221 was significantly down-regulated; furthermore, miR-221 levels were lower in hASC-differentiated adipocytes from obese subjects than in the corresponding adipocytes from lean subjects. Higher TNF-α mRNA levels were associated with lower levels of miR-221. In addition, the miR-221 levels in the adipocytes were inversely correlated with BMI. Conclusion: Our results support the link between miR-221 and obesity development as well as obesity related inflammatory status. Copyright © 2013 S. Karger AG, Basel
- Subjects
MICRORNA; TUMOR necrosis factors; ADIPOSE tissues; MESENCHYMAL stem cells; OVERWEIGHT women; CELL differentiation; ADIPOKINES
- Publication
Cellular Physiology & Biochemistry (Karger AG), 2013, Vol 32, Issue 1, p127
- ISSN
1015-8987
- Publication type
Article
- DOI
10.1159/000350131