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- Title
Molecular Weight Characteristics and Anticoagulant Activity of Low-Molecular-Weight Heparin Obtained By Nitrous Acid Depolymerization.
- Authors
Yur'eva, K. P.; Frumin, L. E.; Baeva, E. A.; Matveev, A. V.; Zhavoronok, E. S.; Prokhorov, D. I.; Grebenkina, L. E.; Panov, A. V.; Kedik, S. A.
- Abstract
Low-molecular-weight heparin was synthesized by depolymerization of high-molecular-weight heparin with nitrous acid. Preparative chromatography was used to isolate low-molecular-weight heparins with narrow molecular-weight distributions, as confirmed by size-exclusion HPLC. The average molecular weight of the products according to capillary viscometry decreased with synthesis time (4 h) from 14.0 to 3.4 kDa. Fractions with molecular-weight characteristics, ratios of sulfo- to carboxyl groups, and specific activities corresponding to those of low-molecular-weight heparin isolated from commercially available Fragmin® were isolated among the products. Low-molecular-weight heparins with characteristics analogous to those of Dalteparin and Nadroparin substances could be obtained depending on the treatment time of high-molecular- weight heparin with nitrous acid in acidic medium.
- Subjects
NITROUS acid; MOLECULAR weights; DEPOLYMERIZATION; LOW-molecular-weight heparin; ANTICOAGULANTS; HIGH performance liquid chromatography; CARBOXYL group
- Publication
Pharmaceutical Chemistry Journal, 2021, Vol 55, Issue 3, p295
- ISSN
0091-150X
- Publication type
Article
- DOI
10.1007/s11094-021-02414-z