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- Title
Protein-crystal detection with a compact multimodal multiphoton microscope.
- Authors
Cheng, Qing-di; Chung, Hsiang-Yu; Schubert, Robin; Chia, Shih-Hsuan; Falke, Sven; Mudogo, Celestin Nzanzu; Kärtner, Franz X.; Chang, Guoqing; Betzel, Christian
- Abstract
There is an increasing demand for rapid, effective methods to identify and detect protein micro- and nano-crystal suspensions for serial diffraction data collection at X-ray free-electron lasers or high-intensity micro-focus synchrotron radiation sources. Here, we demonstrate a compact multimodal, multiphoton microscope, driven by a fiber-based ultrafast laser, enabling excitation wavelengths at 775 nm and 1300 nm for nonlinear optical imaging, which simultaneously records second-harmonic generation, third-harmonic generation and three-photon excited ultraviolet fluorescence to identify and detect protein crystals with high sensitivity. The instrument serves as a valuable and important tool supporting sample scoring and sample optimization in biomolecular crystallography, which we hope will increase the capabilities and productivity of serial diffraction data collection in the future. Cheng, Chung et al. develop a compact multimodal, multiphoton microscope for the detection of protein crystals with high sensitivity. This new tool facilitates the identification and detection of protein micro- and nano-crystal suspensions for serial diffraction data collection at X-ray free-electron lasers or high-intensity microfocus synchrotron radiation sources.
- Subjects
PROTEIN crystallography; MULTIPHOTON processes; WAVELENGTHS; DIFFRACTION patterns; DATA analysis
- Publication
Communications Biology, 2020, Vol 3, Issue 1, pN.PAG
- ISSN
2399-3642
- Publication type
Article
- DOI
10.1038/s42003-020-01275-8