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- Title
HO-1 expression in adipose tissue of obese young SD rats and its role in inflammation and anti-inflammatory mechanism.
- Authors
LIU Tingting; LIU Geli; ZHAO Jing; HE Juan; BAO Pengli; DING Xiaoming
- Abstract
Objective To investigate the change of HO-1 expression in adipose tissue of obese young SD rats as well as its relationship with macrophage infiltration and polarization. Methods Three-week old SD rats (n=24) were randomly divided into 2 groups, routine diet group (NC) and high fat diet group (FC). After feeding 4 weeks, triglyceride (TG), high density lipoprotein (HDL-C), fasting glucose and insulin were compared between these two groups and the insulin resistance index was calculated. The gene expressions of HO-1, IL-6, IL-10 and MCP-1 were assessed by quantitative PCR. Infiltration and polarization of macrophages and M2 macrophages in the visceral adipose tissue were examined by immunohistochemistry. Results The levels of FINS, FBG and HOMA-IR in rats of FC group were higher than those of rats in NC group after 4 weeks feeding (P < 0.05). The level of HO-1, IL-6, MCP-1 in rats from FC group were significantly higher while level of IL-10 were lower compared with those in rats from NC group after 4 weeks of feeding (P < 0.05). In samples from FC groups, more macrophages were detected in adipose tissue by DAB staining than those from NC group. There was no significant difference (P > 0.05) in MOD value of F4/80 and CD206 between these two groups (P > 0.05). Conclusion The infiltration of macrophage in visceral adipose tissue of obese young SD rats significantly increased while HO-1 expression was reactively increased. This insinuated that HO-1 might play an important role in anti-inflammatory mechanism through regulating polarization of macrophages.
- Subjects
HEME oxygenase; ADIPOSE tissues; MACROPHAGES; BLOOD sugar; INSULIN resistance; GENE expression in mammals; LABORATORY rats
- Publication
Tianjin Medical Journal, 2015, Vol 43, Issue 4, p367
- ISSN
0253-9896
- Publication type
Article
- DOI
10.11958/j.issn.0253-9896.2015.04.009