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- Title
Intracellular Calcium Dynamics, Shortened Action Potential Duration, and Late-Phase 3 Early Afterdepolarization in Langendorff-Perfused Rabbit Ventricles.
- Authors
TANG, LIANG; JOUNG, BOYOUNG; OGAWA, MASAHIRO; CHEN, PENG‐SHENG; LIN, SHIEN‐FONG
- Abstract
Calcium Dynamics, APD Shortening, and Late-Phase 3 EAD. Introduction: To elucidate the mechanism of late-phase 3 early after depolarization (EAD) in ventricular arrhythmogenesis, we hypothesized that intracellular calcium (Cai) overloading and action potential duration (APD) shortening may promote late-phase 3 EAD and triggered activity, leading to development of ventricular fibrillation (VF). Methods and Results: In isolated rabbit hearts, we performed microelectrode recording and simultaneous dual optical mapping of transmembrane potential (Vm) and Cai transient on left ventricular endocardium. An IKATP channel opener, pinacidil, was used to abbreviate APD. Rapid pacing was then performed. Upon abrupt cessation of rapid pacing with cycle lengths of 60-200 milliseconds, there were APD90 prolongation and the corresponding Cai overloading in the first postpacing beats. The duration of Cai transient recovered to 50% (DCaT50) and 90% (DCaT90) in the first postpacing beats was significantly longer than baseline. Abnormal Cai elevation coupled with shortened APD produced late-phase 3 EAD induced triggered activity and VF. In additional 6 preparations, the heart tissues were treated with BAPTA-AM, a calcium chelator. BAPTA-AM significantly reduced the maximal Cai amplitude (26.4 ± 3.5% of the control; P < 0.001) and the duration of Cai transients in the mapped region, preventing the development of EAD and triggered activity that initiated VF. Conclusions: I KATP channel activation along with Cai overloading are associated with the development of late-phase 3 EAD and VF. Because acute myocardial ischemia activates the IKATP channel, late-phase 3 EADs may be a mechanism for VF initiation during acute myocardial ischemia. (J Cardiovasc Electrophysiol, Vol. 23, pp. 1364-1371, December 2012)
- Subjects
ACTION potentials; ANIMAL experimentation; CALCIUM; HEART conduction system; RESEARCH methodology; ORGANIC compounds; RABBITS; RESEARCH funding; T-test (Statistics); TISSUE culture; DATA analysis software; CHELATING agents; DESCRIPTIVE statistics; VENTRICULAR arrhythmia
- Publication
Journal of Cardiovascular Electrophysiology, 2012, Vol 23, Issue 12, p1364
- ISSN
1045-3873
- Publication type
Article
- DOI
10.1111/j.1540-8167.2012.02400.x