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- Title
Effects of octacosanol on HMG-CoA reductase and cyclooxygenase-2 activities in the HT-29 human colorectal cancer cell line.
- Authors
Saenthaweesuk, Suphaket; Thaeomor, Atcharaporn; Rabintossaporn, Pornrut; Naowaboot, Jarinyaporn; Somparn, Nuntiya
- Abstract
Octacosanol (OCT) is a major component of policosanol which has been reported to possess antiinflammatory and lipid-lowering effects. Therefore, it was our interest to evaluate the effects of OCT on HMG-CoA reductase (HMGR) and cyclooxygenase-2 (COX-2) activities in a human colorectal cancer cell line (HT-29). Our results demonstrate that 100 µM OCT decreased viability of HT-29 cells as analyzed by sulforhodamine B colorimetric assay with more pronounced effects seen in cells treated with atorvastatin (AST) or celecoxib (CLX), the inhibitors to HMGR and COX-2, respectively. Additionally, the activity of HMGR was found to be inhibited in cells treated with OCT, while COX-2 activity was unaffected; these effects were also more pronounced in AST- and CLX-treated cells. In cells treated with OCT, a significant decrease in HMGR protein expression was observed, but there was no alteration in COX-2 protein expression as determined by Western blot. Taken together, our results show that OCT inhibited HT-29 cell growth and that this effect might be attributed to the reduction of HMGR protein expression and activity.
- Subjects
COLORECTAL cancer; CYCLOOXYGENASE 2; CELL lines; PROTEIN expression; CELL growth; CANCER cells
- Publication
ScienceAsia, 2022, Vol 48, Issue 1, p32
- ISSN
1513-1874
- Publication type
Article
- DOI
10.2306/scienceasia1513-1874.2022.007