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- Title
Effect of recombinant canine interferon-γ on granulocyte-macrophage colony-stimulating factor, transforming growth factor-β and CC chemokine ligand 17 mRNA transcription in a canine keratinocyte cell line (CPEK).
- Authors
Shibata, Sanae; Maeda, Sadatoshi; Kondo, Naho; Inoue, Akiko; Maeda, Shingo; Chimura, Naoki; Fukata, Tsuneo
- Abstract
Recombinant canine interferon-γ (rCaIFN-γ) produced by a baculovirus expression system has therapeutic efficacy against atopic dermatitis in dogs. Although the mechanism of action of rCaIFN-γ is not completely understood, rCaIFN-γ is thought to downregulate the activity of interleukin-4- and interleukin-5-producing T helper 2 cells. However, rCaIFN-γ may also act directly on canine keratinocytes by inhibiting the release of inflammatory mediators. In this study, we investigated the effects of rCaIFN-γ on cytokine and chemokine mRNA transcription in a canine keratinocyte cell line, CPEK. It was found that granulocyte-macrophage colony-stimulating factor (GM-CSF) mRNA transcription was significantly inhibited after treatment with rCaIFN-γ ( P < 0.001), whereas transforming growth factor-β and CC chemokine ligand 17 mRNA levels were unchanged. This study suggests that rCaIFN-γ may suppress GM-CSF production from canine keratinocytes, although further studies are required to confirm this.
- Subjects
INTERFERONS; TRANSFORMING growth factors; CHEMOKINES; KERATINOCYTES; CELL lines; ATOPIC dermatitis; DOG diseases
- Publication
Veterinary Dermatology, 2011, Vol 22, Issue 1, p24
- ISSN
0959-4493
- Publication type
Article
- DOI
10.1111/j.1365-3164.2010.00897.x