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- Title
Chronic alcohol administration alters metabolomic profile of murine bone marrow.
- Authors
Pontes Pereira, Tássia Tatiane; Fideles Duarte-Andrade, Filipe; Gardone Vitório, Jéssica; do Espírito Santo Pereira, Taiane; Braga Martins, Flavia Rayssa; Marques Souza, Jéssica Amanda; Luisa Malacco, Nathália; Mathias Melo, Eliza; Costa Picossi, Carolina Ráıssa; Pinto, Ernani; Santiago Gomez, Ricardo; Martins Teixeira, Mauro; Nori de Macedo, Adriana; Baptista Canuto, Gisele André; Marianetti Soriani, Frederico
- Abstract
Introduction: People with hazardous alcohol use are more susceptible to viral, bacterial, and fungal infections due to the effect of alcohol on immune system cell function. Metabolized ethanol reduces NAD+ to NADH, affecting critical metabolic pathways. Here, our aim was to investigate whether alcohol is metabolized by bone marrow cells and if it impacts the metabolic pathways of leukocyte progenitor cells. This is said to lead to a qualitative and quantitative alteration of key metabolites which may be related to the immune response. Methods: We addressed this aim by using C57BL/6 mice under chronic ethanol administration and evaluating the metabolomic profile of bone marrow total cells by gas chromatography–coupled mass spectrometry (GC–MS). Results: We identified 19 metabolites. Our data demonstrated that chronic ethanol administration alters the metabolomic profile in the bone marrow, resulting in a statistically diminished abundance of five metabolites in ethanoltreated animals: uracil, succinate, proline, nicotinamide, and tyrosine. Discussion: Our results demonstrate for the first time in the literature the effects of alcohol consumption on the metabolome content of hematopoietic tissue and open a wide range of further studies to investigate mechanisms by which alcohol compromises the cellular function of the immune system.
- Subjects
BONE marrow; METABOLOMICS; BONE marrow cells; ALCOHOL drinking; CELL physiology
- Publication
Frontiers in Immunology, 2023, p1
- ISSN
1664-3224
- Publication type
Article
- DOI
10.3389/fimmu.2023.1128352