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- Title
Combining altered levels of effector transcripts in circulating T cells with a marker of endothelial injury is specific for active graft-versus-host disease.
- Authors
Biedermann, B. C.; Tsakiris, D. A.; Gregor, M.; Pober, J. S.; Gratwohl, A.
- Abstract
Summary:Cytotoxic T lymphocytes (CTLs) are important effector cells of graft-versus-host disease (GVHD) and vascular endothelial cells are target cells of allospecific CTL. A combined assessment of T-cell activation and endothelial injury should result in a specific and sensitive test for GVHD. We examined circulating T lymphocytes for effector molecules involved in CTL-mediated endothelial injury. We analyzed CD4 and CD8 T lymphocytes of 24 long-term survivors of allogeneic stem cell transplantation with or without GVHD, and nine healthy, age-matched controls for signs of CTL activation and endothelial injury. IFN-? transcript levels in CD8 T cells were significantly elevated in SCT recipients with GVHD compared to patients without GVHD (767 CD3? units/T cell (376-2050) vs 211 CD3? units/T cell (159-274), P=0.01). Fas ligand transcript levels in CD4 T cells were significantly elevated in SCT recipients without GVHD compared to patients with GVHD (20 CD3? units/T cell (0-78) vs 0 CD3? units/T cell (0-0), P=0.01). Von Willebrand factor plasma levels were high in patients with GVHD, but normal in patients without GVHD (209 (186-254) vs 120 (100-141), P=0.0005). This assessment of T-cell activation and endothelial injury results in a sensitive and specific test to identify patients with active chronic GVHD.Bone Marrow Transplantation (2003) 32, 1077-1084. doi:10.1038/sj.bmt.1704258
- Subjects
ANTINEOPLASTIC agents; T cells; GRAFT versus host disease; GRAFT versus host reaction; BONE marrow transplant complications; ENDOTHELIUM; THERAPEUTICS
- Publication
Bone Marrow Transplantation, 2003, Vol 32, Issue 11, p1077
- ISSN
0268-3369
- Publication type
Article
- DOI
10.1038/sj.bmt.1704258