We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Functionally different PIN proteins control auxin flux during bulbil development in Agave tequilana.
- Authors
Juárez, María Jazmín Abraham; Cárdenas, Rocío Hernández; Villa, José Natzul Santoyo; O'Connor, Devin; Sluis, Aaron; Hake, Sarah; Ordaz-Ortiz, José; Terry, Leon; Simpson, June
- Abstract
In Agave tequilana, reproductive failure or inadequate flower development stimulates the formation of vegetative bulbils at the bracteoles, ensuring survival in a hostile environment. Little is known about the signals that trigger this probably unique phenomenon in agave species. Here we report that auxin plays a central role in bulbil development and show that the localization of PIN1-related proteins is consistent with altered auxin transport during this process. Analysis of agave transcriptome data led to the identification of the A. tequilana orthologue of PIN1 (denoted AtqPIN1) and a second closely related gene from a distinct clade reported as 'Sister of PIN1' (denoted AtqSoPIN1). Quantitative real-time reverse transcription--PCR (RT-qPCR) analysis showed different patterns of expression for each gene during bulbil formation, and heterologous expression of the A. tequilana PIN1 and SoPIN1 genes in Arabidopsis thaliana con- firmed functional differences between these genes. Although no free auxin was detected in induced pedicel samples, changes in the levels of auxin precursors were observed. Taken as a whole, the data support the model that AtqPIN1 and AtqSoPIN1 have co-ordinated but distinct functions in relation to auxin transport during the initial stages of bulbil formation.
- Subjects
PLANT proteins; AUXIN; TEQUILA agave; FLOWER development; BIOLOGICAL transport; REVERSE transcriptase polymerase chain reaction; PLANTS
- Publication
Journal of Experimental Botany, 2015, Vol 66, Issue 13, p3893
- ISSN
0022-0957
- Publication type
Article
- DOI
10.1093/jxb/erv191