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- Title
Characterization of oat (Avena sativa L.) x maize (Zea mays L.) hybrids.
- Authors
Skrzypek, Edyta; Noga, Angelika; Marcińska, Izabela; Czyczyło-Mysza, Ilona; Warchoł, Marzena; Dziurka, Kinga; Kapłoniak, Kamila; Warzecha, Tomasz; Sutkowska, Agnieszka; Idziak-Helmcke, Dominika; Rojek, Magdalena; Hosiawa-Barańska, Marta; Werwińska, Krystyna; Nita, Zygmunt
- Abstract
Wide crossing of cereals with maize is often used to produce doubled haploids (DH). Usually in this method total elimination of maize chromosomes are commonly observed in most cereals, but oat can retain one of more maize chromosomes. The aim of the study was identification, morphological characterization and yield components analysis of the OMA (oat-maize addition) lines. In order to identify maize chromosomes retained in oat genome, oat genomic DNA was isolated from the flag leaf. DNA samples were analyzed by PCR method with using 5'-AAA GAC CTC ACG AAA GGC CCA AGG-3' and 5'-AAA TGG TTC ATG CCG ATT GCA CG-3' primers to detect the presence of maize chromatin. When the whole chromosomes or fragments of maize chromosomes were retained in oat genome, genomic DNA of 500 bp (specific maize retrotransposon fragment, Grande I) was amplified, and detected by electrophoresis. All OMA lines with detected Grande I were analyzed with genomic in situ hybridization (GISH) in order to detect and visualize maize chromosomes. To determine which maize chromosome was present in the OMA lines, DNA amplification using SSR-PCR with primers specific for maize chromosomes was performed. Of all the examined oat lines, in 47 OMA lines retrotransposon Grande I was detected. Twenty-six fertile OMA lines were analyzed with GISH. The whole maize chromosomes as well as a banding pattern probably colocalised with 25S rDNA sites in examined lines were found. Additional hybridization signals found in 2-3 pairs of chromosomes (depending on the analyzed line) might correspond 5S rDNA sequences (in the oat genome these sequences are located on 4 pairs of chromosomes). However, it can not be excluded that these signals came from other maize DNA sequences that have been incorporated into oat chromosomes. Studies based on SSR-PCR enabled the detection of 4, 5 and 6 maize chromosomes in three OMA lines. There were no significant morphological differences between OMA and DH lines. The obtained lines were varied mainly in number of grains and thousand grain weight.
- Subjects
PLANT chromosomes; PLANT hybridization; CORN
- Publication
Cereal Research Communications, 2017, Vol 45, p21
- ISSN
0133-3720
- Publication type
Abstract
- DOI
10.1556/0806.45.2017.100