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- Title
Effects of lyophilization and storage temperature on <italic>Wuchereria bancrofti</italic> antigen sensitivity and stability.
- Authors
Agbozo, Edem Y.; Dumashie, Edward; Boakye, Daniel A.; de Souza, Dziedzom K.
- Abstract
Objective: Antigen-based rapid diagnostic tests for Lymphatic filariasis (LF) do not come with external quality control (QC) materials, and research and disease control programmes rely on stored positive samples. This study was undertaken to evaluate the use of lyophilized <italic>Wuchereria bancrofti</italic> antigen positive plasma samples to serve as QC materials for LF diagnostic tests. 10 well characterized <italic>W. bancrofti</italic> positive samples were lyophilized and stored at 4, 28 and 40 °C. The samples were evaluated using the Alere Filariasis Test Strips before lyophilization, and after 1 and 3 months of storage. The sensitivity and stability of the lyophilized samples were evaluated. Results: The results revealed a loss of sensitivity and stability with increasing temperature and duration of storage. The results are further discussed in terms of the use of dried blood spot (DBS) in diagnostic studies on LF, and the need for thoughtful DBS preparation and storage.
- Subjects
TREATMENT of filariasis; DRIED blood spot testing; BLOOD sampling; PREVENTIVE medicine; FREEZE-drying
- Publication
BMC Research Notes, 2018, Vol 11, Issue 1, pN.PAG
- ISSN
1756-0500
- Publication type
Article
- DOI
10.1186/s13104-018-3586-0