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- Title
Proteomic analysis of Escherichia coli detergent-resistant membranes (DRM).
- Authors
Guzmán-Flores, José E.; Steinemann-Hernández, Lidia; González de la Vara, Luis E.; Gavilanes-Ruiz, Marina; Romeo, Tony; Alvarez, Adrián F.; Georgellis, Dimitris
- Abstract
Membrane microdomains or lipid rafts compartmentalize cellular processes by laterally organizing membrane components. Such sub-membrane structures were mainly described in eukaryotic cells, but, recently, also in bacteria. Here, the protein content of lipid rafts in Escherichia coli was explored by mass spectrometry analyses of Detergent Resistant Membranes (DRM). We report that at least three of the four E. coli flotillin homologous proteins were found to reside in DRM, along with 77 more proteins. Moreover, the proteomic data were validated by subcellular localization, using immunoblot assays and fluorescence microscopy of selected proteins. Our results confirm the existence of lipid raft-like microdomains in the inner membrane of E. coli and represent the first comprehensive profiling of proteins in these bacterial membrane platforms.
- Subjects
ESCHERICHIA coli; LIPID rafts; BACTERIAL proteins; MASS analysis (Spectrometry); MEMBRANE lipids
- Publication
PLoS ONE, 2019, Vol 14, Issue 10, p1
- ISSN
1932-6203
- Publication type
Article
- DOI
10.1371/journal.pone.0223794