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- Title
Moxonidine inhibits Na<sup>+</sup> /H<sup>+</sup> exchange in proximal tubule cells and cortical collecting duct.
- Authors
Schlatter, Eberharid; Ankorina-Stark, Ieva; Haxelmans, Sabine; Hohage, Helge
- Abstract
The imidazoline receptor agonist moxonidine has been recently introduced as an antihypertensive therapy. Imidazoline specific binding sites have also been found in the kidney. Moxonidine induced natriuresis and diuresis in clearance studies in rats. Related substances such as various guanidinium derivatives have been shown to inhibit Na+/H+ exchange in several preparations. We therefore examined whether the renal effects of moxonidine could be mediated by an inhibition of the Na+/H+ exchanger. Intracellular pH (pHi) was measured microfluorimetrically with BCECF in proximal LLC-PK1 cells and in the principal cells of rat cortical collecting ducts (CCD). In LLC-PK1 cells moxonidine (10 μmol/liter) had no effect on the basal pHi, however, it reduced the Na+/H+ activity reversibly by 43 ± 4% (<em>N</em> = 26) when the exchanger was activated by cellular acidification. In rat CCD cells moxonidine slightly decreased basal pHi by 0.08 ± 0.03 pH units (<em>N</em> = 12). After acidification the recovery rate of pHi was reduced with moxonidine by 45 ± 6% (<em>N</em> = 18). The effects of moxonidine could be mimicked in both cell types by inhibitors of the Na+/H+ exchanger (HOE 694, amiloride). In the presence of the imidazoline receptor antagonist idazoxan (10 μmol/liter) the effects of moxonidine were almost completely inhibited. The α2-antagonist yohimbine (10 μmol/liter) did not significantly alter the effects of moxonidine in both cell types. These data suggest that in LLC-PK1 and in rat CCD cells, Na+/H+ is inhibited by moxonidine via an activation of the imidazoline receptor.
- Subjects
BLOOD pressure; CHEMICAL reactions; BIOCHEMISTRY; DIURETICS; NATRIURESIS; FLUORIMETRY
- Publication
Kidney International, 1997, Vol 52, Issue 2, p454
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1038/ki.1997.352