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- Title
A Truncated Retrotransposon Disrupts the GRM1 Coding Sequence in Coton de Tulear Dogs with Bandera's Neonatal Ataxia.
- Authors
Zeng, R.; Farias, F. H. G.; Johnson, G. S.; McKay, S. D.; Schnabel, R. D.; Decker, J. E.; Taylor, J. F.; Mann, C. S.; Katz, M. L.; Johnson, G. C.; Coates, J. R.; O'Brien, D. P.
- Abstract
Bandera's neonatal ataxia (BNAt) is an autosomal recessive cerebellar ataxia that affects members of the Coton de Tulear dog breed. To identify the mutation that causes BNAt. The study involved DNA from 112 Cotons de Tulear (including 15 puppies with signs of BNAt) and 87 DNA samples from dogs of 12 other breeds. The BNAt locus was mapped with a genome-wide association study (GWAS). The coding exons of positional candidate gene GRM1, which encodes metabotropic glutamate receptor 1, were polymerase chain reaction (PCR)-amplified and resequenced. A 3-primer PCR assay was used to genotype individual dogs for a truncated retrotransposon inserted into exon 8 of GRM1. The GWAS indicated that the BNAt locus was in a canine chromosome 1 region that contained candidate gene GRM1. Resequencing this gene from BNAt-affected puppies indicated that exon 8 was interrupted by the insertion of a 5′-truncated retrotransposon. All 15 BNAt-affected puppies were homozygous for the insert, whereas all other Cotons de Tulear were heterozygotes (n = 43) or homozygous (n = 54) for the ancestral allele. None of the 87 dogs from 12 other breeds had the insertion allele. BNAt is caused by a retrotransposon inserted into exon 8 of GRM1. A DNA test for the GRM1 retrotransposon insert can be used for genetic counseling and to confirm the diagnosis of BNAt.
- Subjects
TRANSPOSONS; COTON de Tulear; DOG diseases; ATAXIA; CEREBELLAR ataxia
- Publication
Journal of Veterinary Internal Medicine, 2011, Vol 25, Issue 2, p267
- ISSN
0891-6640
- Publication type
Article
- DOI
10.1111/j.1939-1676.2010.0666.x