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- Title
Incorporated sarcolemmal fish oil fatty acids shorten pig ventricular action potentials
- Authors
Verkerk, Arie O.; van Ginneken, Antoni C.G.; Berecki, Géza; den Ruijter, Hester M.; Schumacher, Cees A.; Veldkamp, Marieke W.; Baartscheer, Antonius; Casini, Simona; Opthof, Tobias; Hovenier, Robert; Fiolet, Jan W.T.; Zock, Peter L.; Coronel, Ruben
- Abstract
Abstract: Background: Omega-3 polyunsaturated fatty acids (ω3-PUFAs) from fish oil reduce the risk of sudden death presumably by preventing life-threatening arrhythmias. Acutely administered ω3-PUFAs modulate the activity of several cardiac ion channels, but the chronic effects of a diet enriched with fish oil leading to ω3-PUFA-incorporation into the sarcolemma on membrane currents are unknown. Methods: Pigs received a diet either rich in ω3-PUFAs or in ω9-fatty acids for 8weeks. Ventricular myocytes (VMs) were isolated and used for patch-clamp studies. Results: ω3-VMs contained higher amounts of ω3-PUFAs and had a shorter action potential (AP) with a more negative plateau than control VM. In ω3 VMs, L-type Ca2+ current (I Ca,L) and Na+–Ca2+ exchange current (I NCX) were reduced by approximately 20% and 60%, respectively, and inward rectifier K+ current (I K1) and slow delayed rectifier K+ current (I Ks) were increased by approximately 50% and 70%, respectively, compared to control. Densities of rapid delayed rectifier K+ current, Ca2+-activated Cl− current, and Na+ current (I Na) were unchanged, although voltage-dependence of I Na inactivation was more negative in ω3 VMs. Conclusions: A fish oil diet increases ω3-PUFA content in the ventricular sarcolemma, decreases I Ca,L and I NCX, and increases I K1 and I Ks, resulting in AP shortening. Incorporation of ω3-PUFAs in the sarcolemma may have consequences for arrhythmias independent of circulating ω3-PUFAs.
- Subjects
HEART diseases; FISH oils; MARINE animal oils; FATTY acids
- Publication
Cardiovascular Research, 2006, Vol 70, Issue 3, p509
- ISSN
0008-6363
- Publication type
Article
- DOI
10.1016/j.cardiores.2006.02.022