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- Title
Restricted γδ T-cell receptor repertoire in IgA nephropathy renal biopsies.
- Authors
Wu, Huiling; Clarkson, Anthony R.; Knight, John F.
- Abstract
Restricted γδ T-cell receptor repertoire in IgA nephropathy renal biopsies. Background. We have previously reported that γδ T cells are involved in the progression of IgA nephropathy (IgAN) to renal failure. Our current study examined the diversity of the CDR3 region of the γδ T-cell receptor (TCR), and characterized the junctional sequences of γδ chain TCR transcripts from T cells infiltrating renal biopsies from patients with IgAN and in peripheral blood T cells (PBLs) from the same patients. Method. RNA extracted from renal biopsies and PBLs of IgAN patients (N = 15) was transcribed and then amplified with primers specific for the four Vγ and six Vδ families. Controls were renal biopsies from thin basement membrane disease (N = 6) and a sample from a kidney with suppurative pyelonephritis. CDR3 length spectratyping and sequencing of TCR γδ-chain were used to analyze the diversity of CDR3 region of these receptors. Results. CDR3 spectratyping of γδ TCR junctional diversity demonstrated that TCR γδ chains (Vγ1-3 and Vδ1-3) expressed by T cells from PBLs of IgAN patients and the infected kidney showed highly diverse junctional lengths that were broadly distributed. In contrast, the junctional lengths of Vγ1 (Vγ2, 3, 4, 5, and 8 genes) and Vδ1 transcripts in the T cells infiltrating kidneys with IgAN were much more restricted than those of PBLs. Renal biopsies from thin basement membrane disease demonstrated no significant signal for any Vγ or Vδ family. Sequence analysis of Vγ1 and Vδ1 transcripts from those patients with restricted CDR3 spectratyping profiles confirmed oligoclonal expansion of γδ T cells infiltrating the kidneys in those IgAN patients and also revealed recurrent junctional amino acid motifs in the TCR Vδ1 chain in the kidney with IgAN. Conclusion. The data show that γδ T cells infiltrating the...
- Subjects
T cell receptors; RENAL biopsy; LYMPHOCYTES
- Publication
Kidney International, 2001, Vol 60, Issue 4, p1324
- ISSN
0085-2538
- Publication type
Article
- DOI
10.1046/j.1523-1755.2001.00937.x