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- Title
Do fetal extravillous trophoblasts circulate in maternal blood postpartum?
- Authors
Looij, Anne; Singh, Ripudaman; Hatt, Lotte; Ravn, Katarina; Jeppesen, Line D.; Nicolaisen, Bolette H.; Kølvraa, Mathias; Vogel, Ida; Schelde, Palle; Uldbjerg, Niels; van de Looij, Anne
- Abstract
<bold>Introduction: </bold>Circulating fetal extravillous trophoblasts may offer a superior alternative to cell-free fetal DNA for noninvasive prenatal testing. Cells of fetal origin are a pure source of fetal genome; hence, unlike the cell-free noninvasive prenatal test, the fetal cell-based noninvasive prenatal test is not expected to be affected by maternal DNA. However, circulating fetal cells from previous pregnancies may lead to confounding results.<bold>Material and Methods: </bold>To study whether fetal trophoblast cells persist in maternal circulation postpartum, blood samples were collected from 11 women who had given birth to a boy, with blood sampling at 1-3 days (W0), 4-5 weeks (W4-5), around 8 weeks (W8) and around 12 weeks (W12) postpartum. The existence of fetal extravillous trophoblasts was verified either by X and Y chromosome fluorescence in situ hybridization analysis or by short tandem repeat analysis. To exclude technological bias in isolating fetal cells, blood samples were also collected from 10 pregnant women between a gestational age of 10 and 14 weeks, the optimal time frame for cell-based noninvasive prenatal test sampling. All the samples were processed according to protocols established by ARCEDI Biotech for fetal extravillous trophoblast enrichment and isolation.<bold>Results: </bold>Fetal extravillous trophoblasts were found in all the 10 samples from pregnant women between a gestational age of 10 and 14 weeks. However, only 4 of 11 blood samples taken from women at 1-3 days postpartum rendered fetal extravillous trophoblasts, and only 2 of 11 samples rendered fetal extravillous trophoblasts at 4 weeks postpartum.<bold>Conclusions: </bold>In this preliminary dataset on few pregnancies, none of the samples rendered any fetal cells at or after 8 weeks postpartum, showing that cell-based noninvasive prenatal testing based on fetal extravillous trophoblasts is unlikely to be influenced by circulating cells from previous pregnancies.
- Subjects
SHORT tandem repeat analysis; FLUORESCENCE in situ hybridization; CHORIONIC villus sampling; Y chromosome; PRENATAL diagnosis; RESEARCH; TROPHOBLAST; DNA; CYTOMETRY; RESEARCH methodology; EVALUATION research; COMPARATIVE studies; PUERPERIUM; QUESTIONNAIRES; MENTAL health surveys; RESEARCH funding; POLYMERASE chain reaction
- Publication
Acta Obstetricia et Gynecologica Scandinavica, 2020, Vol 99, Issue 6, p751
- ISSN
0001-6349
- Publication type
journal article
- DOI
10.1111/aogs.13880