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- Title
Auricle shaping using 3D printing and autologous diced cartilage.
- Authors
Liao, Junlin; Chen, Yong; Chen, Jia; He, Bin; Qian, Li; Xu, Jiaqin; Wang, Aijun; Li, Qingfeng; Xie, Hongju; Zhou, Jianda
- Abstract
<bold>Objective: </bold>To reconstruct the auricle using a porous, hollow, three-dimensional (3D)-printed mold and autologous diced cartilage mixed with platelet-rich plasma (PRP).<bold>Methods: </bold>Materialise Magics v20.03 was used to design a 3D, porous, hollow auricle mold. Ten molds were printed by selective laser sintering with polyamide. Cartilage grafts were harvested from one ear of a New Zealand rabbit, and PRP was prepared using 10 mL of auricular blood from the same animal. Ear cartilage was diced into 0.5- to 2.0-mm pieces, weighed, mixed with PRP, and then placed inside the hollow mold. Composite grafts were then implanted into the backs of respective rabbits (n = 10) for 4 months. The shape and composition of the diced cartilage were assessed histologically, and biomechanical testing was used to determine stiffness.<bold>Results: </bold>The 3D-printed auricle molds were 0.6-mm thick and showed connectivity between the internal and external surfaces, with round pores of 0.1 to 0.3 cm. After 4 months, the diced cartilage pieces had fused into an auricular shape with high fidelity to the anthropotomy. The weight of the diced cartilage was 5.157 ± 0.230 g (P > 0.05, compared with preoperative). Histological staining showed high chondrocyte viability and the production of collagen II, glycosaminoglycans, and other cartilaginous matrix components. In unrestricted compression tests, auricle stiffness was 0.158 ± 0.187 N/mm, similar to that in humans.<bold>Conclusion: </bold>Auricle grafts were constructed successfully through packing a 3D-printed, porous, hollow auricle mold with diced cartilage mixed with PRP. The auricle cartilage contained viable chondrocytes, appropriate extracellular matrix components, and good mechanical properties.<bold>Levels Of Evidence: </bold>NA. Laryngoscope, 129:2467-2474, 2019.
- Subjects
NEW Zealand; THREE-dimensional printing; CARTILAGE; SELECTIVE laser sintering; PLATELET-rich plasma; CARTILAGE transplantation; ANIMALS; BIOLOGICAL models; COMPUTER-aided design; EAR; EXTERNAL ear; RABBITS; PLASTIC surgery
- Publication
Laryngoscope, 2019, Vol 129, Issue 11, p2467
- ISSN
0023-852X
- Publication type
journal article
- DOI
10.1002/lary.27752