We found a match
Your institution may have access to this item. Find your institution then sign in to continue.
- Title
Establishment of a Homologous Silencing System with Intact-Plant Infiltration and Minimized Operation for Studying Gene Function in Herbaceous Peonies.
- Authors
Zhang, Kaijing; Wang, Xiaobin; Chen, Xiaoxuan; Zhang, Runlong; Guo, Junhong; Wang, Qiyao; Li, Danqing; Shao, Lingmei; Shi, Xiaohua; Han, Jingtong; Liu, Zhiyang; Xia, Yiping; Zhang, Jiaping
- Abstract
Gene function verification is a crucial step in studying the molecular mechanisms regulating various plant life activities. However, a stable and efficient homologous genetic transgenic system for herbaceous peonies has not been established. In this study, using virus-induced gene silencing technology (VIGS), a highly efficient homologous transient verification system with distinctive advantages was proposed, which not only achieves true "intact-plant" infiltration but also minimizes the operation. One-year-old roots of the representative species, Paeonia lactiflora Pall., were used as the materials; prechilling (4 °C) treatment for 3–5 weeks was applied as a critical precondition for P. lactiflora to acquire a certain chilling accumulation. A dormancy-related gene named HOMEOBOX PROTEIN 31 (PlHB31), believed to negatively regulate bud endodormancy release (BER), was chosen as the target gene in this study. GFP fluorescence was detected in directly infiltrated and newly developed roots and buds; the transgenic plantlets exhibited remarkably earlier budbreak, and PlHB31 was significantly downregulated in silenced plantlets. This study established a homologous transient silencing system featuring intact-plant infiltration and minimized manipulation for gene function research, and also offers technical support and serves as a theoretical basis for gene function discovery in numerous other geophytes.
- Subjects
PLANT gene silencing; PEONIES; HOMEOBOX proteins; HOMEOBOX genes; GENE silencing; BUDS; GENES
- Publication
International Journal of Molecular Sciences, 2024, Vol 25, Issue 8, p4412
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms25084412