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- Title
ZnO Tetrapods for Label-Free Optical Biosensing: Physicochemical Characterization and Functionalization Strategies.
- Authors
Terracciano, Monica; Račkauskas, Simas; Falanga, Andrea Patrizia; Martino, Sara; Chianese, Giovanna; Greco, Francesca; Piccialli, Gennaro; Viscardi, Guido; De Stefano, Luca; Oliviero, Giorgia; Borbone, Nicola; Rea, Ilaria
- Abstract
In this study, we fabricated three different ZnO tetrapodal nanostructures (ZnO-Ts) by a combustion process and studied their physicochemical properties by different techniques to evaluate their potentiality for label-free biosensing purposes. Then, we explored the chemical reactivity of ZnO-Ts by quantifying the available functional hydroxyl groups (–OH) on the transducer surface necessary for biosensor development. The best ZnO-T sample was chemically modified and bioconjugated with biotin as a model bioprobe by a multi-step procedure based on silanization and carbodiimide chemistry. The results demonstrated that the ZnO-Ts could be easily and efficiently biomodified, and sensing experiments based on the streptavidin target detection confirmed these structures' suitability for biosensing applications.
- Subjects
TETRAPODS; HYDROXYL group; FUNCTIONAL groups; SILANIZATION; SURFACE analysis
- Publication
International Journal of Molecular Sciences, 2023, Vol 24, Issue 5, p4449
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms24054449