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- Title
Association of IL-10 gene (−1082A>G, −819C>T and −592C>A) polymorphism and its serum level with metabolic syndrome of north Indian subjects.
- Authors
MADESHIYA, AMIT; SINGH, SHRADDHA; DWIVEDI, SHIPRA; KONWAR, RITURAJ; NATU, SHANKAR; GHATAK, ASHIM
- Abstract
Metabolic syndrome (MetS) is an inflammatory disorder, in which various cytokines play important role in tilting balance towards disease state. Interleukin-10 ( IL-10) is an important antiinflammatory cytokine, but its genetic polymorphisms and serum levels in Indian MetS subjects are unknown. Three IL-10 gene polymorphisms (−1082A >G (rs1800896), −819C >T (rs1800872) and −592C >A (rs1800871)) were genotyped with PCR-RFLP in MetS subjects ( n = 384) and age/sex matched control subjects ( n = 386). Serum IL-10 was measured using enzyme-linked immunosorbent assay. Serum IL-10 level was significantly low in MetS subject and significantly correlated with clinicobiochemical parameters of MetS. Of three investigated promoter polymorphisms, IL-10 -819C > T and -592C >A were significantly associated with risk of MetS. The mutant alleles −819T and −592A of IL-10 gene polymorphism were significantly higher in MetS subjects compared to controls. Of the four different haplotypes obtained, common ACC haplotype and rare GTA haplotype of IL-10 polymorphisms were associated with MetS. The mean of fasting insulin and HOMA-IR were significantly different between the genotypes of both −819 C >T and −592C >A polymorphisms of IL-10 in MetS subjects. These results suggested that polymorphisms in IL-10 gene (−819C >T and −592C >A), haplotypes (ACC and GTA) and serum level are significantly associated with risk of MetS. IL-10 −819C >T and −592C >A polymorphic variants are also significantly associated with insulin level and homeostasis model assessment-insulin resistance in north Indian MetS subjects.
- Subjects
METABOLIC syndrome diagnosis; INTERLEUKIN-10; GENETIC polymorphisms; THERAPEUTIC use of cytokines; INSULIN resistance
- Publication
Journal of Genetics, 2017, Vol 96, Issue 1, p53
- ISSN
0022-1333
- Publication type
Article
- DOI
10.1007/s12041-016-0738-7