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- Title
The expression of HIF-1<sub>α</sub> in ischemic reperfused myocardium treated with postconditioning.
- Authors
Xiao-Ping Lv; Xiao-Liang Wang; Li Yah; Liang Wang; Ye Wu; Xin-Liang Ma; Hui-Rong Liu
- Abstract
Objective: Cardioprotection with postconditioning has been well demonstrated after a short period of reperfusion. This study was designed to investigate whether hypoxia inducible factor-1α (HIF-1α), the major transcription factor and key regulator of adoptive responses to hypoxia, was expressed during myocardial ischemia postconditioning (MIP). Methodes: Male adult rats undergoing 45 min left anterior descending artery occlusion were divided into MI/R (myocardial ischemia and reperfusion) group with 3 h of full reperfusion and MIP group with three l0 s cycles of reperfusion and re-occlusion applied at the onset of 3 h of reperfusion. The expression of HIF-1α was examined by immunohistochemistry and western blot. Myocardial infarct size was identified by Evans blue and TTC staining. Cardiomyocyte apoptosis was qualitatively analyzed by terminal dUTP nick end-labeling (TUNEL) assay. Result: Compared with MI/R group, treatment with MIP reduced myocardial infarct size (16.3±1.7% vs. 27.1±2.3%, P<0.05), decreased cardiomyocyte apoptosis (10.2±2.0% vs.18.2±3.7%, P<0.05). The expression of HIF-1α was significant increased with 1.4-fold in MIP groups versus MI/R groups. Conclusion: MIP has a protective effect on ischemic reperfused myocardium and the effect is intimately related to upregulation of HIF-1α.
- Subjects
HYPOXEMIA; ISCHEMIA; REPERFUSION injury; CORONARY disease; TRANSCRIPTION factors; LABORATORY rats
- Publication
FASEB Journal, 2007, Vol 21, Issue 6, pA1222
- ISSN
0892-6638
- Publication type
Article