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- Title
Effect of the dietary compound fisetin on nuclear factor-kappa B (NF-κB) signaling in HT-29 colon cancer cells.
- Authors
Mi Ra Seon; Han Jin Cho; Il-Jun Kang; Hye-Mi Kim; Seung-Kyoung Jeung; Park, Jung H. Y.
- Abstract
Flavonoids have been reported to have ability to prevent cancer development. Fisetin, a naturally occurring flavonol, is commonly found in fruits, vegetables, and wine. We have previously observed that fisetin inhibits HT-29 human colon cancer cell growth. To examine the effect of fisetin on the NF-κB signaling that is known to play an important role in cell survival and apoptosis, HT-29 cells were treated with 0 or 60 µmol/L fisetin for 10 min, 30 min or 120 min. Western blot analysis revealed that fisetin increased p-IκBα protein levels at 10 and 30 min of culture, and decreased IκBα protein levels after 30 min treatment. Immunoprecipitation and in vitro kinase assay revealed that fisetin dose-dependently increased IκB kinase (IKK) activity. The subcellular fractionation and immunocytochemistry assay revealed that fisetin increased translocation of p65 (Re1A) to the nucleus. In addition, electrophoretic mobility shift assay indicated that fisetin increased NF-κB DNA binding activity in a concentration-dependent manner. These results raised the possibility that fisetin may inhibit HT-29 cell growth via its ability to upregulate NF-κB signaling.
- Subjects
FLAVONOIDS; NF-kappa B; CANCER cells; COLON cancer; WESTERN immunoblotting; CHROMOSOMAL translocation
- Publication
FASEB Journal, 2007, Vol 21, Issue 5, pA360
- ISSN
0892-6638
- Publication type
Article