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- Title
Comparative Proteomic Analysis of Two Ralstonia solanacearum Isolates Differing in Aggressiveness.
- Authors
Wang, Guoping; Kong, Jie; Cui, Dandan; Zhao, Hongbo; Zhao, Puyan; Feng, Shujie; Zhao, Yahua; Wang, Wenyi
- Abstract
<italic>Ralstonia solanacearum</italic> is a soil-borne, plant xylem-infecting pathogen that causes the devastating bacterial wilt (BW) disease in a number of plant species. In the present study, two <italic>R. solanacearum</italic> strains with different degrees of aggressiveness―namely RsH (pathogenic to Hawaii 7996, a tomato cultivar resistant against most strains) and RsM (non-pathogenic to Hawaii 7996) were identified. Phylogenetic analysis revealed that both RsM and RsH belonged to phylotype I. To further elucidate the underlying mechanism of the different pathotypes between the two strains, we performed a comparative proteomics study on RsM and RsH in rich and minimal media to identify the change in the level of protein abundance. In total, 24 differential proteins were identified, with four clusters in terms of protein abundance. Further bioinformatics exploration allowed us to classify these proteins into five functional groups. Notably, the pathogenesis of RsM and RsH was particularly characterized by a pronounced difference in the abundance of virulence- and metabolism-related proteins, such as UDP-N-acetylglucosamine 2-epimerase (epsC) and isocitrate lyase (ICL), which were more abundant in the high pathogenicity strain RsH. Thus, we propose that the differences in pathogenicity between RsM and RsH can possibly be partially explained by differences in extracellular polysaccharide (EPS) and glyoxylate metabolism-related proteins.
- Subjects
RALSTONIA solanacearum; BACTERIAL wilt diseases; PLANT species; TOMATOES; GLYCOASPARAGINASE
- Publication
International Journal of Molecular Sciences, 2018, Vol 19, Issue 8, p2444
- ISSN
1661-6596
- Publication type
Article
- DOI
10.3390/ijms19082444