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- Title
Mycosynthesis of Size-Controlled Silver Nanoparticles through Optimization of Process Variables by Response Surface Methodology.
- Authors
SHAHZAD, ASMA; IQTEDAR, MEHWISH; SAEED, HAMID; HUSSAIN, SYED ZAJIF; CHAUDHARY, ASMA; ABDULLAH, ROHEENA; KALEEM, AFSHAN
- Abstract
The present study was carried out to reduce the size of silver nanoparticles (AgNPs) by optimizing physico-chemical conditions of the Aspergillus fumigatus BTCB10 growth based on central composite design (CCD) through response surface methodology (RSM). Variables such as a concentration of silver nitrate (mM), NaCl (%) and the wet weight of biomass (g) were controlled to produce spherical, monodispersed particles of 33.23 nm size, observing 78.7% reduction in size as compared to the initially obtained size that was equal to 356 nm. The obtained AgNPs exhibited negative zeta potential of -9.91 mV with a peak at 420 nm in the UV-Vis range whereas Fourier Transform Infrared (FT-IR) analysis identified O-H, C = C, C = C, C-Br and C-Cl groups attached as capping agents. After conducting RSM experiments, a high nitrate reductase activity value of 179.15 nmol/h/ml was obtained; thus indicating a likely correlation between enzyme production and AgNPs synthesis. The F-value (significant at 3.91), non-significant lack of fit and determination coefficient (R2 = 0.7786) is representative of the good relation between the predicted values of response. We conclude that CCD is an effective tool in obtaining significant results of high quality and efficiency.
- Subjects
SILVER nanoparticles; RESPONSE surfaces (Statistics); SILVER nitrate; FOURIER transform infrared spectroscopy; ASPERGILLUS fumigatus
- Publication
Polish Journal of Microbiology, 2019, Vol 68, Issue 1, p35
- ISSN
1733-1331
- Publication type
Article
- DOI
10.21307/pjm-2019-004