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- Title
Characterization of lacosamide metabolites by UHPLC–ESI–HRMS method.
- Authors
Gawlik, Maciej; Skibiński, Robert; Komsta, Łukasz
- Abstract
In this study, the in vitro phase I metabolism of lacosamide was characterized with the use of ultra-high-performance liquid chromatography combined with high-resolution mass spectrometry (quadrupole time-of-flight). The use of two metabolism simulation techniques (photocatalysis and human liver microsomes) allowed the characterization of a polar metabolite of parent compound, not yet described. The experiment with the participation of HLM gave the ability to describe the full liver metabolic pathway of lacosamide. It has been proven that this molecule undergoes deacetylation, demethylation, and during liver tissue metabolism. Photocatalysis with the use of a TiO2 catalyst was proved to be a complementary technique in mimicking in vitro drug metabolism.
- Subjects
TISSUE metabolism; DRUG metabolism; METABOLITES; MASS spectrometry; LIVER microsomes; DEACETYLATION
- Publication
Acta Chromatographica, 2020, Vol 32, Issue 2, p107
- ISSN
1233-2356
- Publication type
Article
- DOI
10.1556/1326.2019.00591