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- Title
The BK channel accessory β<sub>1</sub> subunit determines alcohol-induced cerebrovascular constriction
- Authors
Bukiya, Anna N.; Liu, Jianxi; Dopico, Alejandro M.
- Abstract
Abstract: Ethanol-induced inhibition of myocyte large conductance, calcium- and voltage-gated potassium (BK) current causes cerebrovascular constriction, yet the molecular targets mediating EtOH action remain unknown. Using BK channel-forming (cbv1) subunits from cerebral artery myocytes, we demonstrate that EtOH potentiates and inhibits current at lower and higher than ∼15μM, respectively. By increasing cbv1’s apparent -sensitivity, accessory BK β1 subunits shift the activation-to-inhibition crossover of EtOH action to <3μM , with consequent inhibition of current under conditions found during myocyte contraction. Knocking-down KCNMB1 suppresses EtOH-reduction of arterial myocyte BK current and vessel diameter. Therefore, BK β1 is the molecular effector of alcohol-induced BK current inhibition and cerebrovascular constriction.
- Subjects
POTASSIUM channels; ALCOHOL; MUSCLE cells; CALCIUM; CEREBROVASCULAR disease; VASOCONSTRICTION; CEREBRAL arteries; SALINE solutions
- Publication
FEBS Letters, 2009, Vol 583, Issue 17, p2779
- ISSN
0014-5793
- Publication type
Article
- DOI
10.1016/j.febslet.2009.07.019