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- Title
An integrated genetic and cytogenetic map for the Mediterranean fruit fly, Ceratitis capitata , based on microsatellite and morphological markers.
- Authors
Antonios Augustinos; Yannis Petalas; Michael Vrahatis; Anastasios Mintzas; Antigone Zacharopoulou
- Abstract
Abstract A genetic map based on microsatellite polymorphisms and visible mutations of the Mediterranean fruit fly (medfly), Ceratitis capitata is presented. Genotyping was performed on single flies from several backcross families. The map is composed of 67 microsatellites and 16 visible markers distributed over four linkage groups. Fluorescence in situ hybridization of selected microsatellite markers on salivary gland polytene chromosomes allowed the alignment of these groups to the second, fourth, fifth and sixth chromosome. None of the markers tested showed segregation either with the X or the third chromosome. However, this map constitutes a substantial starting point for a detailed genetic map of C. capitata. The construction of an integrated map covering the whole genome should greatly facilitate genetic studies and future genome sequence projects of the species.
- Subjects
GENETICS; GENETIC polymorphisms; CELL nuclei; CHROMOSOMES
- Publication
Genetica, 2008, Vol 133, Issue 2, p147
- ISSN
0016-6707
- Publication type
Article