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- Title
Me1<sub>1b</sub> receptor mediated action of melatonin in regulation of lung associated immune system (LAIS) of Perdicula asiatica: An in vitro study.
- Authors
Kharwar, Rajesh Kumar; Haldar, Chandana; Singh, Sweta
- Abstract
The pineal hormone melatonin plays an important role in immunomodulation of avian species. These immunomodulatory effects of melatonin are known to be mediated by three membrane bound receptors found in birds, Mel1a, Mel1b and Mel1c- However, specific involvement of these receptors in mediating immunomodulatory effects of melatonin in Lung Associated Immune System (LAIS) remains unexplored till date. The aim of the present study was to check the specific melatonin receptor subtype involved in regulation of LAIS. For this purpose, we used luzindole, a non selective and 4P-PDOT, a selective Mel1b receptor antagonist under in vitro condition to assess their specific potency towards melatonin induced cellular immunity in the lung tissue of Perdicula asiatica. Melatonin enhanced proliferation of lymphocytes isolated from lung tissue and up-regulated both receptor types (Mel1a and Mel1b) on physiological doses while higher doses decreased the proliferation and down regulated the expression of both receptors. Luzindole decreased proliferation of lung lymphocytes and down-regulated the expression of both receptors in a dose dependent manner while 4P-PDOT decreased proliferation of lymphocytes isolated from lung and down-regulated the expression of Mel1b receptor subtype only. Proliferation rate of lymphocytes isolated from lung tissue followed more the expression pattern of Mel1b than the Mel1a. Thus, we may suggest that Mel1b might be involved in cell mediated immunity in lung of P. asiatica to a greater extent than Mel1a.
- Publication
Biologia, 2015, Vol 70, Issue 11, p1537
- ISSN
0006-3088
- Publication type
Article
- DOI
10.1515/biolog-2015-0177